|
|
|
Mitochondria Isolation Kit for Tissue  |
 |
|
|
|
Mitochondria Isolation Kit for Tissue
- Quick and Convenient - isolates intact mitochondria in less than 60 minutes
- Versatile - offers two methods of isolation from both soft and hard tissue samples
- Multi-sample format - reagent-based approach enables simultaneous processing of multiple samples
- Optional alternate method - reagents and protocol included for the traditional Dounce homogenization procedure with fewer required strokes
- Bench-top compatible - both procedures can be performed using a microcentrifuge tube
|
 |
The Pierce Mitochondria Isolation Kit for Tissue is a novel tool for isolation of intact mitochondria from soft and hard tissue samples. The kit offers two methods for separation of mitochondria. The first method utilizes a unique reagent based procedure that enables simultaneous multi-sample processing. The second method relies on traditional Dounce homogenization for tissue disruption and subsequent isolation of the organelle. Both procedures rely on differential centrifugation to separate the intact mitochondria using a bench-top microcentrifuge and are completed in less than 60 minutes. In addition, both procedures have been optimized for maximum yield of mitochondria with minimal damage to the integrity of the organelle. The isolated mitochondria may be used for a number of downstream applications, including 2D/MS for proteomics research, disease profiling, and metabolic studies.
|
COX4 |
VDAC |
Cytochrome C |
|
M |
C |
M |
C |
M |
C |
| Reagent-based |
 |
| Dounce-based |
 |
Figure 1 - Analysis of mitochondrial integrity. Mitochondria and cytosolic fractions were prepared from fresh rat liver and heart tissue samples using the reagent-based and Dounce homogenization methods. Fractions were analyzed via Western Blot for VDAC, COX4 and Cytochrome C. SuperSignal West Pico Chemiluminescent Substrate (Product# 34080) was used for detection. VDAC (Voltage-dependent Anion Channel) is a membrane protein found on the outer membrane of the mitochondria, COX4 is an inner mitochondria membrane protein, and Cytochrome C resides in the intermembrane space. M: mitochondria, C: Cytosol. |
Related products:
Mitochondrial Isolation Kit for Cultured Cells
U.S. Patent pending on the Mitochondria Isolation Kit Technology. SuperSignal Technology is protected by U.S. Patent #6,432,662. BCA Technology is protected by U.S. Patent # 4,839,295.
Ordering Information
|
 Certificate of Analysis  Product Instructions  MSDS |
| Buy |
Product # |
Description |
Pkg. Size |
Files |
Price |
|
Add
|
89801 |
Mitochondria Isolation Kit for Tissue
| Sufficient reagents for 50 applications. | | Kit contains: | | Mitochondria Isolation Reagent A | 50 ml | | Mitochondria Isolation Reagent B | 500 µl | | Mitochondria Isolation Reagent C | 65 ml | | Bovine Serum Albumin | 230 mg | | BupH Phosphate Buffered Saline | 1 pack | |
kit |
|
$217.00 |
|
|
|
|
|
|
|
|
|
