Target in-cell crosslinking and cell/tissue fixing applications with methanol-free 16% Formaldehyde Solution (w/v) in ampules

High-purity, methanol-free formaldehyde is now offered in convenient application-ready packaging. The solution is carefully prepared directly from granular paraformaldehyde and sealed under an inert atmosphere in 1 ml and 10 ml ampules. Methanol-free formaldehyde is widely used to crosslink molecules in protein-protein and protein-nucleic acid interaction discovery, bioimaging and flow cytometry.

Why Formaldehyde?
Formaldehyde is a highly reactive, cell permeable agent that is used by researchers as a reversible crosslinking agent for proteins and nucleic acids within the cell or as a general cell-fixing agent for imaging-based applications. Cell permeability allows the reagent to readily enter living cells enabling intracellular applications, such as protein interaction discovery or bioimaging. Formaldehyde produces reversible crosslinks with protein and nucleic acids by coupling primary amines that are within proximity. Crosslinks can be reversed simply by heating the sample.

Application-targeted ampule packaging

The package sizes of the 16% Formaldehyde Solution (formalin) are targeted to both low and high volume users. The appropriate package size is selected based on the most common applications requiring a fresh, methanol-free formaldehyde solution. The 16% (w/v) solution simplifies the dilution for each respective application and the application-directed packaging minimizes waste.

• 1 ml ampules are intended for protein-based in cell applications such as Chromatin Immunoprecipitation (ChIP) or protein interaction discovery.
• 10 ml ampules are intended for bioimaging (high content screening/analysis) such as fixing cells on a culture plate before image analysis.

"Prepared fresh" Sealed in Ampules
Our formaldehyde solution is prepared as a 16% (w/v) solution of paraformaldehyde (pure granular solid) in distilled/deionized water. Solution stabilization is important because formaldehyde solutions oxidize to formic acid and eventually repolymerize to paraformaldehyde. Most protocols requiring methanol-free formaldehyde suggest that the formaldehyde be "prepared fresh". This formaldehyde solution is sealed in ampules immediately after formulation under an inert atmosphere. Ampule packaging stabilizes the preparation allowing access to "fresh" formaldehyde each time. Within the amber ampules the formaldehyde solution is protected from both air oxidation and light. The availability of an ampuled solution saves valuable time and eliminates the need and hazards associated with preparing the solution directly from paraformaldehyde.

• Fresh – formaldehyde solution remains stable and free of contamination in an amber pre-scored ampule.
• Sized Right – package sizes geared for applications ranging from culture-targeted cell fixing to in-cell protein-based applications.
• Convenient Starting Concentration – 16% w/v formaldehyde solution simplifies dilution to the required working concentration.
• Minimizes Waste – 1 ml and 10 ml package sizes allow selection of product that will result in less waste and minimize disposal concerns.

• Cell/Tissue Fixing for Imaging
• In-cell Crosslinking
• Chromatin Immunoprecipitation Assays (ChIP assays)
• Flow Cytometry
• In vivo capture of physiologically relevant interactions (enables capture of both stable and transient interactions)
• Membrane and cytosolic protein complex analysis
• Crosslinking and detection of protein complexes
• Co-immunoprecipitation of complexes after crosslinking
• Western blotting and detection of crosslinked complexes

1. Kiernan, J.A. (2000). Formaldehyde, formalin, paraformaldhyde and glutaraldehyde: What they are and what they do? Microscopy Today 00-1, 8-12.

Ordering Information	Bulk quantities: Inquire    Certificate of Analysis   Product Instructions   MSDS
Product # Description Pkg. Size Files Price 28906 16% Formaldehyde (w/v), Methanol-free 10 x 1ml ampule $27.00 28908 16% Formaldehyde (w/v), Methanol-free 10 x 10ml ampule $41.00