Thermo Scientific Pierce Protein Precipitation Plates for easy target molecule isolation

The study of small molecules and how they behave in complex biofluids (serum, plasma, blood and urine) is an essential part of pharmaceutical, DMPK and metabolomics research. Drugs or other small molecules are routinely monitored for clearance rates, steady-state concentrations, serum-binding equilibrium or in vivo modification. These studies require isolation of target molecules from the protein matrix before analysis. Precipitating proteins with acetonitrile and then removing the precipitate by centrifugation or filtration is the typical isolation process. Although centrifugation to form pellets is inexpensive and reliable, 96-well filtration plates provide an easy format for reproducible, simple, rapid and automatable protein precipitation and sample clarification.

• Prepare small molecule samples for HPLC or LC-MS in 10-30 minutes
• 96-well format for automatable protein precipitation and filtration
• Optimized for parallel processing and automation
• Works seamlessly with the Rapid Equilibrium Device (RED) for plasma protein-binding assays
• No acetonitrile leakage for up to four hours
• Leach-free graded hydrophobic frit prevents blockage
• Filtrates are easily collected by vacuum, positive pressure or centrifugation
• Compatible with most organic solvents

Our 96-well filtration plates are designed specifically for protein precipitation applications. The plate design provides many advantages over standard protein precipitation based on filtration or centrifugation methods. The Thermo Scientific Protein Precipitation Plates contain a special graded filter system that retains and removes protein precipitate particles without clogging, yet ultimately filters eluates through a 0.2 µm cut-off membrane for maximum protein removal. These plates are leak-free and retain organic solvent without dripping, even while mixing. Samples are rapidly recovered by applying vacuum or other pressure. The 2 ml well volume allows processing sample volumes of 15-400 ml per well, using the recommended precipitation conditions of 3:1 (v/v) solvent-to-serum ratio. The Protein Precipitation Plates are the ideal choice for downstream processing of plasma protein binding assay samples prepared with the popular RED Rapid Equilibrium Dialysis System.

Ordering Information	Certificate of Analysis   Product Instructions   MSDS
Buy Product # Description Pkg. Size Files Price Add 90036 Protein Precipitation Plates 2 plates $150.00 Add 90037 Protein Precipitation Plates 10 plates $670.00