Column and spin cup kits for purifying GST fusion proteins from bacteria.

Thermo Scientific B-PER GST Fusion Protein Purification Kits offer rapid purification of GST and GST-tagged fusion proteins from bacteria, yielding up to four times as much protein as traditional methods.

The kits use Thermo Scientific B-PER Bacterial Protein Extraction Reagent to gently and efficiently lyse E. coli cells to extract soluble proteins. The B-PER Lysate is directly loaded on the pre-packed glutathione agarose column or incubated as a slurry in a spin cup. After washing to remove non-GST components of the lysate, the purified GST or GST fusion protein is eluted with reduced free glutathione.

Two kit formats provide the range of scale and convenience required for typical laboratory applications. The column kit purifies approximately 10mg of overexpressed GST protein per use in less than 3 hours. The Spin Kit purifies approximately 1mg of overexpressed GST protein in 30 minutes.

Column Purification Kit Highlights:

• Capacity – purify 10mg of overexpressed GST fusion protein from 250mL bacterial culture
• Purity – yields GST fusion protein free of cellular contamination
• Speed – progress from cell lysis to purified protein in less than two hours
• Easy and efficient cell lysis – no sonication required; complete cell lysis achieved with B-PER Bacterial Protein Extraction Reagent
• Ready-to-use columns and reagents – pre-packed columns (contain 1mL glutathione agarose), prepared wash buffers and easy-to-prepare glutathione solution for elution make set-up and execution very easy
• Convenient size – five columns and sufficient reagents for five purifications

Spin Purification Kit Highlights:

• Speed – progress from E. coli cell culture to purified protein in less than 30 minutes!
• Purity – yields GST fusion protein free of cellular contamination
• Capacity – purify 1mg of overexpressed GST fusion protein from 250mL bacterial culture
• Easy and efficient cell lysis – no sonication required; complete cell lysis achieved with B-PER Bacterial Protein Extraction Reagent
• Ready-to-use components and reagents – microcentrifuge spin cups, glutathione agarose slurry, prepared wash buffer and easy-to-prepare glutathione solution for elution make set-up and execution very easy
• Convenient size – 16 spin cups and sufficient reagents for 16 purifications

Related Products:

Pierce Glutathione Agarose and GST Purification Kits – Recommended!
B-PER Bacterial Protein Extraction Reagents