High purity TFA in 1 ml ampules or 500 ml bottles
...make fresh 0.1% TFA solutions in seconds!
Trifluoroacetic acid (TFA) is the most commonly used ion pairing agent for use in reverse-phase HPLC peptide separations because it...
- sharpens peaks and improves resolution
- is volatile and easily removed
- has low absorption within detection wavelengths
- has a proven history
Trifluoroacetic Acid Highlights:
- High purity and exceptional clarity – allows sensitive, nondestructive peptide detection at low UV wavelengths in reverse-phase HPLC protein and peptide separation systems
- High-performance packaging – TFA packaged under nitrogen in amber glass ampules or bottles with protective PTFE-lined fluorocarbon caps to ensure TFA integrity
- Economical convenience – Choose the TFA format that works best for your application. In just a few seconds, 1 ml ampules can be used to prepare 1 liter of fresh 0.1% v/v trifluoroacetic acid solution for the mobile phase in reverse-phase chromatography
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| Properties of Trifluoroacetic Acid |
| Molecular formula |
CF3COOH |
| Molecular weight |
114.03 |
| Density |
1.53 g/ml, 20°C |
| Melting/boiling point |
-15°C / 72°C |
| Alternative names |
Perfluoroacetic acid, Trifluoroethanoic acid, Trifluoracetic acid |
| CAS Number |
76-05-01 |
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Trifluoroacetic Acid Specifications
Thermo Scientific Pierce TFA is manufactured to the highest specifications to ensure the integrity of your data, maximize sensitivity in your assay and to prolong the life of your equipment. Each lot of trifluoroacetic acid is tested to meet the following minimum specifications:
- TFA Purity: > 99.5%
- Water content: < 0.1%
- Chain length: > 99.5% C2
- Ninhydrin positives: A570 < 0.02 above blank
- Tollen’s test (aldehydes): Negative
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- UV Absorbance (0.1% aqueous)
- A280 < 0.002
- A254 < 0.005
- A230 < 0.090
- UV Absorbance (neat)
- A300 < 0.03
- A275 < 0.04
- Cut-off < 262 nm
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Applications for Trifluoroacetic Acid
References
- Chicz, R.M. and Regnier, F.E. (1990) Methods Enzymol. 182, 392-421.
- Zarember, K.A., et al. (2002) Infect. Immun. 70, 569-576.
- Lassy, R.A. and Miller, C.G. (2000) J. Bacteriol. 182, 2536-2543.
- Smith, B.J. (1997) Protein Sequencing Protocols. Humana Press.
- Allen, G. (1989) Sequencing of Proteins and Peptides, Second Revised Addition. Elsevier.
- Backstrom, J.R., et al. (1996) J. Neurosci. 16, 7910-7919.
- Hermann, P.M., et al (2000) J. Neurosci. 20, 6355-6364.
- Stuart, J.M. and Young, J.D. (1984) Solid Phase Peptide Synthesis, Second Edition. Pierce Chemical Company.
* Trifluoroacetic Acid is available in bulk quantities for manufacturing applications.
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Bulk quantities: Inquire
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