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BupH Dry Buffers  |
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The most advanced, versatile, time-saving buffer productline available.
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 BupH Packs are pre-blended and pre-measured dry mixtures of commonly needed buffers that are easy to prepare; simply empty contents of foil envelope pack into a beaker, add ultrapure water (or water and methanol for BupH Tris-Glycine Transfer Buffer), and stir to dissolve. The packs eliminate weighing time and tedious pH adjustments. BupH Pack Dry Blend Buffers are offered for use in common laboratory techniques and to support other Pierce protein research products. Formulations are fully disclosed, so if you need to, you can make these and other buffers from scratch using the recipes that follow the BupH Pack ordering information table. |
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The ultimate in convenience …
- Reach for the sealed foil pack sitting conveniently on your bench top.
- Open and add water.
- The fresh buffer is ready to use in practical aliquots so there’s no waste.
The ultimate in versatility …
- Routine buffers are designed for use in dialysis, crosslinking, enzyme assays, ELISAs, immuno-histochemistry, protein plate-coating, biotinylation and other applications.
- Using one buffer source maintains consistency and eliminates variables within the lab.
- Specialized buffers ideally support your work in specific chemistries and methods.
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The ultimate in integrity …
- Unlike stored buffers, BupH Buffers are protected from contamination.
- Carry out applications with confidence in buffer quality.
- "Test-assured" with the Pierce commitment to world-class, quality management standards.
The ultimate in time savings …
- The making of specialized and routine buffers is no longer time-consuming.
- No component measurement, pH adjustment, quality validation, preparation tracking or refrigeration hassles.
- Move forward with your work by eliminating re-tests due to buffer problems.
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Buffer Pack
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Application
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| BupH Borate Buffer Packs |
Ideal for protein modification procedures that require an alkaline pH. |
| BupH Carbonate-Bicarbonate Buffer Packs |
Ideal for microplate coating for RIA and EIA techniques. |
| BupH MES Buffered Saline Packs |
Ideal for microplate coating for RIA and EIA techniques Ideal for use with carbodiimide coupling chemistries. |
| BupH Modified Dulbecco’s PBS Packs |
A ready-to-use PBS for immunoassays. |
| BupH Phosphate Buffered Saline Packs |
Its ionic strength makes it ideal for crosslinking
and biotinylation. |
| BupH Tris-Glycine and Tris Buffered Saline |
Great for Western blots! |
| Tris-Glycine-SDS Buffer Packs |
A ready-to-use electrophoresis buffer. |
* BupH Dry Buffers are available in bulk quantities for manufacturing applications.
Related Products and Links:
Concentrated Buffers – 10x and 20x stock solutions for making up to 10 liters of buffer
Desalting and Buffer Exchange Columns and Plates
Dialysis Cassettes and Tubing
Ordering Information
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 Certificate of Analysis  Product Instructions  MSDS |
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Product # |
Description |
Pkg. Size |
Files |
Price |
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28372 |
BupH Phosphate Buffered Saline Packs
Each pack yields 500 ml of 0.1 M phosphate, 0.15 M NaCl, pH 6.9 - 7.2 when dissolved in 500 ml distilled water (20 liters total) |
40 packs |
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$126.00 |
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28374 |
BupH Modified Dulbecco's PBS Packs
Each pack yields 500 ml of 0.008 M sodium phosphate, 0.002 M potassium phosphate, 0.14 M sodium chloride and 0.01 M potassium chloride, pH 7.4 when dissolved in 500 ml distilled (20 liters total) |
40 packs |
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$90.00 |
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28376 |
BupH Tris Buffered Saline Packs
Each pack yields 500 ml of 25 mM Tris, 0.15 M NaCl, pH 7.2 when dissolved in 500 ml distilled water (20 liters total) |
40 packs |
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$126.00 |
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28378 |
BupH Tris-Glycine-SDS Buffer Packs
Each pack yields 500 ml of 25 mM Tris, 192 mM Glycine and 0.1% SDS, pH 8.3 when dissolved in 500 ml distilled water (20 liters total) |
40 packs |
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$111.00 |
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28379 |
BupH Tris Buffered Saline Packs
Each pack yields 500 ml of 25 mM Tris, 0.15 M NaCl, pH 7.2 when dissolved in 500 ml distilled water (5 liters total). |
10 packs |
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$57.00 |
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28380 |
BupH Tris-Glycine Buffer Packs
Each pack yields 500 ml of 25mM Tris and 192 mM Glycine at a pH of approximately 8 when dissolved in 400 ml distilled water and 100 ml of methanol (20 liters total) |
40 packs |
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$112.00 |
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28382 |
BupH Carbonate-Bicarbonate Buffer Packs
Each pack yields 0.2M Carbonate-Bicarbonate Buffer, pH 9.4 when dissolved in 500 ml distilled water (20 liters total). |
40 packs |
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$117.00 |
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28384 |
BupH Borate Buffer Packs
Each pack yields 50 mM borate, pH 8.5 after adding 500 ml of deionized water (20 liters total). |
40 packs |
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$124.00 |
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28386 |
BupH Citrate-MOPS Buffer Packs
Each pack yields 0.6 M sodium citrate, 0.1 M MOPS buffer, pH 7.5 when dissolved in 100 ml of deionized water (1 liter total) |
10 packs |
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$54.00 |
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28388 |
BupH Citrate-Carbonate Buffer Packs
Each pack yields 0.6 M sodium citrate, 0.1 M sodium carbonate, pH 9, when dissolved in 100 ml of ultrapure water (1 liter total) |
10 packs |
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$59.00 |
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28390 |
BupH MES Buffered Saline Packs
Each pack yields 500 ml of 0.1 M 2-[morpholino]ethanesulfonic acid, 0.9% NaCl, pH 4.7 when dissolved in 500 ml deionized water (5 liters total) |
10 packs |
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$104.00 |
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28398 |
BupH Tris-HEPES-SDS Running Buffer
Each pack yields 500 ml of 100 mM Tris, 100 mM HEPES, 3 mM SDS, pH 8 ± 0.5 when dissolved in 500 ml distilled water
(5 liters total). |
10 packs |
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$29.00 |
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