Highlights:

• Water insoluble
• Non-cleavable
• Popular for forming enzyme immunoconjugates
• Reactive groups: NHS ester and maleimide
• Reactive towards: amino and sulfhydryl groups
• Sulfonated, water-soluble analog also available; see Sulfo-MBS;
  see also: SMPB.

References:

1. Chrisey, L.A., et al. (1996). Nucleic Acids Res. 24(15), 3031-3039.
2. Myers, D.E., Uckun, F.M., Swaim, S.E. and Vallera, D.A. (1989). The effects of aromatic and aliphatic maleimide crosslinkers and anti-CD-5 ricin immunotoxins. J. Immunol. Meth. 121, 129-142. (MBS example)
3. Kitagawa, T. and Aikawa, T. (1976). Enzyme coupled immunoassay of insulin using a novel coupling reagent. J. Biochem. (Tokyo) 79, 233-236.