A complete selection of purified, conjugated and activated streptavidin proteins for nearly any biotin-binding assay technique.

Streptavidin is biotin-binding protein that was originally isolated from Streptomyces avidinii. In contrast to avidin, streptavidin has no carbohydrate and has a mildly acidic pI of 5. Thermo Scientific Pierce Streptavidin products use a recombinant form of streptavidin having a mass of 53,000 daltons and a near-neutral pI. Streptavidin is a tetrameric protein, with each subunit binding one molecule of biotin with affinity similar to that of avidin. Guanidinium chloride will dissociate avidin and streptavidin into subunits, but streptavidin is more resistant to dissociation.

Purified streptavidin and most of the conjugates are supplied lyophilized in phosphate-based buffers and may be reconstituted with water. Once reconstituted, these products are handled and stored similar to other proteins and enzyme conjugates (see the Tech Tip: Protein stability and storage). Alkaline Phosphatase conjugates are supplied as a liquid in a Tris-based buffer.

Applications for enzyme-labeled (HRP, AP) streptavidin

• Enzyme immunoassays (EIA)
• Cell and tissue staining (for light microscopy)
• Western blotting (WB) and other membrane-based biotin detection
• Also see our line of High Sensitivity Streptavidin Conjugates, which provide "poly-HRP" sensitivity and performance in many applications.

• Immunofluorescent assays (IFA)
• Tissue staining (for fluorescent microscopy)
• Blot immunostaining
• Cell staining (for fluorescent microscopy and activated cell sorting)
• Also see our complete line of DyLight Fluor Streptavidin Conjugates, which replace and surpass the performance of traditional fluorescein and rhodamine fluors in many fluorescence applications.

Related Products
High Sensitivity Streptavidin HRP Conjugates
High Stabilized (Pre-diluted) Streptavidin HRP Conjugates
DyLight Fluor Streptavidin Conjugates
Streptavidin Agarose Resin
NeutrAvidin Protein and Conjugates

References

1. Chaiet, I. and Wolf, F.J. (1964). Arch. Biochem. Biophys. 106, 1-5.
2. Savage, M.D., et al. (1992). Avidin-Biotin Chemistry: A Handbook. Rockford, Illinois: Pierce Chemical Company.
3. Wilchek, M. and Bayer, E.A. (1983). Anal. Biochem. 71, 1-32.
4. Zuk, P.A. and Elferink, L.A. (2000). J. Biol. Chem. 275, 26754-26764.
5. Conti, L.R., et al. (2001). J. Biol. Chem. 276, 41270-41278.