Resins with high biotin affinity and low nonspecific binding.

Streptavidin is a tetrameric protein containing four biotin-binding sites similar to avidin. The native protein isolated from culture filtrates of Streptomyces avidinii is carbohydrate-free, has an acidic pI of 5.5 and a mass of 75 kDa. Streptavidin generally has less nonspecific binding than avidin (from chicken egg white) because of the absence of carbohydrates.

Our Streptavidin Resins (i.e., Immobilized Streptavidin Products) use a recombinant form of streptavidin with a mass of 53 kDa and a near-neutral pI. These resins are prepared by covalently coupling streptavidin to the porous agarose or UltraLink Biosupport using efficient and stable chemistries, resulting in Streptavidin Agarose and UltraLink Resins that are leach-resistant and stable at pH 2-11. The products are excellent choices for a variety of small- or large-scale affinity purification applications involving biotinylated macromolecules, including separation of biotinylated molecules from samples and immunoprecipitation of antigens using biotin-labeled antibodies.

Highlights:

• Proven coupling chemistries – The methods used to prepare these resins are superior to typical cyanogen bromide (CNBr) immobilization, providing high functionality, low nonspecific binding and excellent leach-resistance.
• Versatile resins – Agarose (polysaccharide-based) and UltraLink Biosupport (acrylamide-based) resins provide two different options for particular experimental conditions and are compatible with gravity-flow, spin and FPLC methods.
• Available in multiple formats – Choose from two resin types, regular or high capacity preparations, and small or large-scale package sizes (bulk sizes are also available).
• Superior performance – High Capacity Resin has 2-3 times higher binding capacity than alternative supplier products, allowing use of small amounts of resin for experiments (see streptavidin data).
  

Applications:

• Purification of membrane antigens in conjunction with biotinylated monoclonal antibodies^1,2
• Cell-surface labeling with biotinylation reagents, followed by precipitation with immobilized streptavidin³
• Purification of cell-surface glycoproteins using biotinylated Concanavalin A ⁴
• Recovery of single-stranded biotinylated DNA for dideoxy sequencing ⁵
• Streptavidin-coated plates used for direct solid-phase sequencing ^6,7
• In vitro mutagenesis with streptavidin-coated plates ⁸
• DNA fingerprinting with streptavidin-coated plates ⁹

Related Products:

NeutrAvidin Agarose and UltraLink Resins
Avidin Agarose Resin
Pierce Monomeric Avidin Resins and Kit
Purified Streptavidin and Streptavidin Conjugates
Streptavidin Coated Plates
Pierce Streptavidin Magnetic Beads

References:

1. Updyke, T.V. and Nicolson, G.L. (1984). Immunoaffinity isolation of membrane antigens with biotinylated monoclonal antibodies and immobilized streptavidin matrices. J. Immunol. Methods.73, 83-95.
2. Buckie, J.W. and Cook, G.M.W. (1986). Specific isolation of surface glycoproteins from intact cells by biotinylated concanavalin A and immobilized streptavidin. Anal. Biochem.156, 463-472.
3. Gretch, D.R., et al. (1987). The use of biotinylated monoclonal antibodies and streptavidin-affinity chromatography to isolate herpes virus hydrophobic proteins or glycoproteins. Anal. Biochem.163, 270-277.
4. Lisanti, M.P., et al. (1989). Steady -state distribution and biogenesis of endogenous Madin-Darby canine kidney glycoproteins: evidence for intracellular sorting and polarized cell surface delivery. J. Cell Biol.109, 2117-2127.
5. Mitchell, L.G. and Merril, C.R. (1989). Affinity generation of single-stranded DNA for dideoxy sequencing following the polymerase chain reaction. Anal. Biochem.178, 239-242.
6. Hultman, T., et al. (1989). Direct solid phase sequencing of genomic DNA using magnetic beads as solid support Nucleic Acids Research 17(13), 4937-4946.
7. Hultman, T., et al. (1990). Solid phase in vitro mutagenesis using plasmid DNA template. Nucleic Acids Research 18(17), 5107-5112.
8. Hultman, T., et al. (1991). Bidirectional solid-phase sequencing of in vitro-amplified plasmid DNA. Biotechniques 10(1), 84-93.
9. Stacy, J.E., et al. (1991). Fingerprinting of diverse species with DNA probes generated from immobilized single-stranded DNA templates. Nucleic Acids Research 19(14), 4004.