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Protein G Agarose and UltraLink Resins |
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High binding, low leaching supports for antibody purification and immunoprecipitation.
| Thermo Scientific Pierce Protein G Agarose and UltraLink Resins are effective for affinity purification of IgG from serum and other fluids of many species. Protein G is especially suited for purification of monoclonal antibodies from mouse and the broadest spectrum of species and IgG subclasses from human, goat and sheep samples. Our Protein G Agarose and UltraLink Resins use a recombinant form of Protein G immobilized using chemistries that are leak-resistant, providing high-capacity affinity supports with minimal nonspecific binding. These agarose and polyacrylamide supports can be regenerated and reused multiple times when stored properly. |
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Protein G Beads - Quick Reference
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| Protein G |
Recombinant protein expressed in E. coli (21.6 kDa; two IgG binding sites) |
Specificity
(see details) |
Best for IgG from mouse, human, cow, goat and sheep; poor for guinea pig, pig, dog and cat |
Supports
Offered |
Crosslinked 6% beaded agarose
UltraLink Biosupport
Magnetic particles (1-4 µm) |
Package
Formats |
Resin slurries (3 sizes)
Chromatography Cartridges (2 sizes)
Packed Spin Columns (3 sizes)
Nab IgG Purification Kits (2 sizes)
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| Storage |
4°C, do not freeze |
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Protein G is a bacterial cell wall protein original from group G Streptococci and now produced as a recombinant in E. coli. Like Protein A, Protein G binds to most mammalian immunoglobulins primarily through their Fc regions. Native Protein G contains two immunoglobulin binding sites, as well as albumin and cell surface binding sites. In the recombinant form of Protein G, these albumin and cell surface binding sites have been eliminated to reduce nonspecific binding when purifying immunoglobulins. Recombinant Protein G has a mass of approximately 21.6 kDa. However, its apparent size by SDS-PAGE is 31-34 kDa.
Protein G Resin Highlights:
- Efficient and leach-resistant immobilization chemistries provide for high capacity and purity for antibody purification
- Albumin and cell surface binding site have been removed from recombinant Protein G to provide higher antibody purity
- Binds to a wider range of antibodies than Protein A beads
- Available in regular and high ("Plus") binding-capacity formats
- Offered as resin slurries, chromatography cartridges, NAb Spin Columns and Purification Kits
(Learn more about Pierce Chromatography Cartridges or about NAb Spin Columns.)
Choose between Protein A, Protein G, Protein L:
Related Products:
References:
- Bjorck, L. and Kronvall, G. (1984) J. Immunol. 133, 969-974.
- Guss, B., et al. (1986) EMBO J. 15, 1567-1575.
- Eliasson, M., et al. (1988) J. Biol. Chem. 263, 4323-4327.
- Lévy, F., et al. (2005) Mol. Biol. Cell. 16, 1777-1787.
- Petrucelli, L., et al. (2004) Hum. Mol. Genet. Apr 2004; 13, 703-714.
- Rissman, R.A., et al. (2004) J. Clin. Invest. 114, 121-130.
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Ordering Information
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 Bulk quantities: Inquire  Certificate
of Analysis  Product
Instructions  MSDS |
| Product # |
Description |
Pkg. Size |
Files |
Price |
| 89926 |
Pierce Protein G Chromatography Cartridges, 1mL
Learn more about Pierce Chromatography Cartridges. |
2 cartridges |
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$175.00
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| 89927 |
Pierce Protein G Chromatography Cartridges, 5mL
Learn more about Pierce Chromatography Cartridges. |
1 cartridge |
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$351.00
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| 20398 |
Pierce Protein G Agarose
Support: Crosslinked 6% beaded agarose
Capacity: 11-15mg human IgG/mL of resin
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2mL |
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$199.00
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| 20399 |
Pierce Protein G Agarose
Support: Crosslinked 6% beaded agarose
Capacity: 11-15mg human IgG/mL of resin |
10mL |
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$740.00
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| 20397 |
Pierce Protein G Agarose
Support: Crosslinked 6% beaded agarose
Capacity: 11-15mg human IgG/mL of resin |
25mL |
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$1,625.00
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| 89953 |
NAb Protein G Spin Columns, 0.2mL |
10 columns |
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$243.00
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| 89957 |
NAb Protein G Spin Columns, 1mL |
5 columns |
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$442.00
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| 89961 |
NAb Protein G Spin Columns, 5mL |
1 column |
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$409.00
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| 89949 |
NAb Protein G Spin Kit, 0.2mL
Kit contents:
NAb Protein G Spin Columns, 0.2mL, 10 columns
Microcentrifuge Tubes, 2mL, 80 tubes
PBS Pack (makes 500mL), 1 pack
IgG Elution Buffer (pH 2.8), 50mL
Neutralization Buffer, 12mL
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10-column kit |
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$299.00
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| 89979 |
NAb Protein G Spin Kit, 1mL
Kit contents:
NAb Protein G Spin Columns, 1mL, 2 columns
PBS Pack (makes 500mL), 1 pack
IgG Elution Buffer (pH 2.8), 240mL
Neutralization Buffer, 12mL
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2-column kit |
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$356.00
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| 22851 |
Pierce Protein G Plus Agarose
Support: Crosslinked 6% beaded agarose
Capacity: > 20mg human IgG/mL of resin |
2mL |
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$250.00
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| 22852 |
Pierce Protein G Plus Agarose
Support: Crosslinked 6% beaded agarose
Capacity: > 20mg human IgG/mL of resin |
10mL |
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$859.00
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| 53125 |
Protein G UltraLink Resin
Support: Ultralink Biosupport
Capacity: > 20mg of human IgG/mL of resin
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2mL |
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$258.00
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| 53126 |
Protein G UltraLink Resin
Support: UltraLink Biosupport
Capacity: > 20mg human IgG/mL of resin |
10mL |
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$877.00
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| 53127 |
Protein G UltraLink Columns, 2mL |
2 columns |
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$510.00
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| 53128 |
Protein G Plus UltraLink Resin
Support: UltraLink Biosupport
Capacity: > 25mg of human IgG/mL of resin |
2mL |
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$335.00
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| 21193 |
Pierce Recombinant Protein G |
5mg |
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$290.00
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