Fluorescent detection of glycoproteins

The Krypton Glycoprotein Staining Kit provides a fast and easy method for the fluorescent detection of glycoproteins in sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and 2-D gels (Figure 1). The stain exhibits sensitivity equivalent to, or greater than, other fluorescent glycoprotein stains and uses well-established periodate-oxidation chemistry that preferentially (15- to 20-fold more binding to glycoproteins) reacts with glycoproteins (Figure 2).

Highlights:

• Excitation/emission maxima – 654/673 nm
• Multiplex-compatible – after glycoprotein staining, gels can be stained with Krypton Protein Stain or colorimetric stains
• Robust – highly consistent, reproducible glycoprotein staining
• Linear quantitative range – 3 orders of magnitude
• Sensitive – detect down to 15 ng of glycoprotein
• Fast – total protocol time of 4 hours
• Compatible – well-suited to work with mass spec analysis and common imaging systems

Figure 1. Krypton Glycoprotein Stain works up to four times faster than other fluorescent glycoprotein stain protocols.

Figure 2. Krypton Glycoprotein Stain provides a fast and sensitive method for detecting glycoproteins in polyacrylamide gels. The gel was stained with Krypton Glycoprotein Stain followed by total protein staining with Krypton Protein Stain (Product # 46630). Lanes 1-6 contain a mixture of the indicated proteins at the following concentrations: Lane 1: 500 ng, Lane 2: 250 ng,
Lane 3: 125 ng,  Lane 4: 63 ng, Lane 5: 31 ng and Lane 6: 15 ng.