Lumi-Phos WB Chemiluminescent Substrate overcomes all of the limitations posed by conventional chemiluminescent substrates for AP. Lumi-Phos WB Substrate provides sensitivity in the low picogram range, enabling you to detect mere attomoles of your target ligand. Lumi-Phos WB also produces less background noise than other popular chemiluminescent substrates for AP, providing a better signal:noise ratio and a clearer image. Because signal generation is immediate, there’s
no need to wait 15 to 30 minutes for a measurable signal. All of these benefits are combined with a low, low price.

Highlights:

• High sensitivity – able to detect 1.2 pg or 71 attomoles of the target
  ligand mouse IL-2
• Low background – high signal:noise ratios produce clearer blots
• Inexpensive – less expensive than CDP-Star Substrate (based on 2008 U.S. list prices), and there is no need to purchase additional enhancers for nitrocellulose membranes
• Long signal duration – allows you to redevelop blots over and over
• Immediate strong signal – no more waiting 15 to 30 minutes for the signal to become strong enough to detect
• Ready to use – no mixing required with this one-component system

Figure 1. Attomole-range detection and crystal-clear background.
Serial dilutions of recombinant mouse IL-2 were separated on a 4%-20% SDS polyacrylamide gel, then transferred to nitrocellulose membrane. The membranes were subsequently incubated in a
1 µg/ml dilution of purified rat anti-mouse IL-2, followed by a 200 ng/ml dilution of AP-labeled goat anti-rat IgG. The membranes were washed and then incubated in Lumi-Phos WB Substrate for five minutes prior to film exposure.

References:

1. Bruinsma, P., et al. (2004). J. Biol. Chem. 279, 39814-39823.
2. Dhingra, A., et al. (2004). Proc. Nat. Acad. Sci. USA 101, 6315-6320.
3. Tikhonov, I., et al. (2003). J. Virol. 77, 3157-3166.