Page: 04-L-Protein Assay Compatibility Table


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Protein Assay Compatibility Table

Protein assay methods are based on different dye-binding and chemical reactions with protein functional groups. These differences contribute to different sensitivities to other chemicals components that may be present in protein assay sample.

The following table summarizes the compatibilities of common laboratory sample components with Thermo Scientific Pierce Protein Assays. The values represent the maximum compatible concentration of substances in the protein sample. Substances were tested individually in BSA samples and were considered compatible if they affected the measurement value less than 10%.

Use this information only as a guideline for choosing an appropriate protein assay or for evaluating the possible source of abberant results. Actual samples, which normally contain several different buffer and other components, may behave differently.

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Summary of Substance Compatibilities with Thermo Scientific Pierce Protein Assays.
	Thermo Scientific Pierce Protein Assay (names link to product pages)
Substance	BCA	BCA - RAC	Micro BCA	Modified Lowry	660 nm (see note)	Coomassie (Bradford)	Coomassie Plus
Detergents	(<)	(<)	(<)	(<)	(<)	(<)	(<)
Tween - 20	5%	10%	5%	0.062%	10%	0.062%	0.031%
Triton X-114	1%	2%	0.05%	0.031%	0.5%	0.125%	0.062%
Triton X-100	5%	10%	5%	0.031%	1%	0.125%	0.062%
CHAPS	5%	10%	1%	0.062%	5%	5%	5%
SDS	5%	10%	5%	1%	0.01% (5%)	0.125%	0.016%
OTG	5%	10%	5%	n/a	5%	3%	3%
Zwittergent 3-14	1%	2%	n/c	n/a	0.05%	0.025%	0.025%
Reducing Agents	(<)	(<)	(<)	(<)	(<)	(<)	(<)
Dithiothreitol (DTT)	1 mM	5 mM	n/c	n/c	500 mM	5 mM	5 mM
2-Mercaptoethanol	1 mM	35 mM	1 mM	1 mM	1 M	1 M	1 M
TCEP	n/c	10 mM	n/c	n/a	40 mM	n/a	n/a
Chelating Agents	(<)	(<)	(<)	(<)	(<)	(<)	(<)
EDTA	10 mM	20 mM	0.5 mM	1 mM	20 mM	100 mM	100 mM
Sodium citrate	200 mM	100 mM	5 mM	0.1 mM	12.5 mM	200 mM	200 mM
Buffers/Salts	(<)	(<)	(<)	(<)	(<)	(<)	(<)
Tris	100 mM	50 mM	50 mM	10 mM	250 mM	2 M	2 M
HEPES, pH 7.5	100 mM	200 mM	100 mM	1 mM	100 mM	100 mM	100 mM
MES, pH 6.1	100 mM	100 mM	100 mM	125 mM	125 mM	100 mM	100 mM
Imidazole, pH 7.0	50 mM	50 mM	12.5 mM	25 mM	200 mM	200 mM	200 mM
Guanidine-HCl	4 M	2 M	4 M	n/a	2.5 M	3.5 M	3.5 M
Urea	3 M	4 M	3 M	3 M	8 M	3 M	3 M
Sucrose	40%	40%	4%	7.5%	50%	10%	10%
n/a = not assayed n/c = not compatible Note: The Pierce 660 nm Assay is compatible with 5% SDS when used with the optional IDCR component available with the kit. Refer to individual product instructions for more comprehensive compatibility information.

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